spike proteins  (Sino Biological)

Journal: Applied Microbiology and Biotechnology

Article Title: A broadly applicable split-luciferase biosensor approach for rapid antibody detection in emerging infectious diseases

doi: 10.1007/s00253-026-13712-5

Figure Lengend Snippet: Sensor pair selection for detection of SARS-CoV-2 and CHIKV antibodies. a Different sensor combinations were screened for their ability to reconstitute luciferase activity in the presence of anti-SARS-CoV-2 RBD antibodies. b Sensor pairs were similarly evaluated for luciferase complementation in the presence of anti-CHIKV E2 antibodies. Data represent means of duplicate tests; error bars indicate standard deviations

Article Snippet: Plates were coated with SARS-CoV-2 spike RBD ( P08128 , GenBank accession no. NC_045512 ; Solarbio, China) or CHIKV E2 (strain SL-CK1; 40,440-V08B; GenBank accession no. ADG95913 ; Sino Biological, China) at 0.1 μg/well at 4 °C overnight, washed with PBST, and blocked with 2% BSA for 1 h at 37 °C.

Techniques: Selection, Luciferase, Activity Assay

Journal: Applied Microbiology and Biotechnology

Article Title: A broadly applicable split-luciferase biosensor approach for rapid antibody detection in emerging infectious diseases

doi: 10.1007/s00253-026-13712-5

Figure Lengend Snippet: Analytical performance of the bioluminescent immunoassay for detection of anti-SARS-CoV-2 RBD (6.39 mg/mL) and anti-CHIKV E2 antibodies (1.0 mg/mL). Data represent means of duplicate tests; error bars indicate standard deviations

Article Snippet: Plates were coated with SARS-CoV-2 spike RBD ( P08128 , GenBank accession no. NC_045512 ; Solarbio, China) or CHIKV E2 (strain SL-CK1; 40,440-V08B; GenBank accession no. ADG95913 ; Sino Biological, China) at 0.1 μg/well at 4 °C overnight, washed with PBST, and blocked with 2% BSA for 1 h at 37 °C.

Techniques:

Journal: npj Viruses

Article Title: K5 polysaccharides inhibit SARS-CoV-2 infection by preventing spike-proteolytic priming

doi: 10.1038/s44298-025-00163-4

Figure Lengend Snippet: a Representative images of syncytia formation assay in VeroE6 cells upon treatment (350 µM) with K5 compounds. Scale bar: 50 µm. b Number of nuclei involved in syncytia formation is higher in Wuhan-Hu-1 spike-positive cells than in Omicron BA.1 spike-positive cells. c Effect of K5 compounds on syncytia formation induced by Wuhan-Hu-1 spike. d Effect of K5 compounds on syncytia formation induced by Omicron BA.1 spike. Only spike-positive cells were quantified. Data of four (Wuhan-Hu-1) and three (Omicron BA.1) independent experiments. Values are mean ± sd. P values determined by Welch’s t-test: * P < 0.05; ** P < 0.005.

Article Snippet: Reagents and materials were used as received, unless otherwise mentioned, and were purchased from the following: Human recombinant SARS-CoV-2 Wuhan-Hu-1 spike His-Tag protein and RBD from Sino Biological (#40592-V08B); ACE2 from Acrobiosystem (#AC2-H52H8); Bovine Serum Albumin (BSA) from Merck (#810037); Human recombinant furin from OriGene Technologies Inc. (#TP304279M); Conventional heparin (13.6 kDa - purity ≥95%) from a commercial batch of unfractionated sodium heparin from Laboratori Derivati Organici S.p.A. (#9041-08-1).

Techniques: Tube Formation Assay

Journal: npj Viruses

Article Title: K5 polysaccharides inhibit SARS-CoV-2 infection by preventing spike-proteolytic priming

doi: 10.1038/s44298-025-00163-4

Figure Lengend Snippet: VeroE6 cells or A549 ACE2+ cells were treated with increasing concentrations of heparin, K5, K5OSH, and K5NOSH. a , b The cells remained viable in the presence of heparin and the K5 compounds as evaluated by measuring the ATP levels. VeroE6 or A549 ACE2+ cells were infected with the B.1 c , d or Omicron BA.1 e , f isolates in the presence or the absence of increasing concentrations of heparin, K5, K5OSH, and K5NOSH. Infection was reduced in a concentration-dependent manner as shown by the percentage of plaque reduction compared to SARS-CoV-2 alone. Data are presented as the mean value ± standard error of three independent replicates. * P < 0.05; ** P < 0.005.

Article Snippet: Reagents and materials were used as received, unless otherwise mentioned, and were purchased from the following: Human recombinant SARS-CoV-2 Wuhan-Hu-1 spike His-Tag protein and RBD from Sino Biological (#40592-V08B); ACE2 from Acrobiosystem (#AC2-H52H8); Bovine Serum Albumin (BSA) from Merck (#810037); Human recombinant furin from OriGene Technologies Inc. (#TP304279M); Conventional heparin (13.6 kDa - purity ≥95%) from a commercial batch of unfractionated sodium heparin from Laboratori Derivati Organici S.p.A. (#9041-08-1).

Techniques: Infection, Concentration Assay