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sars-cov-2 (2019-ncov) nucleocapsid-his recombinant protein  (Sino Biological)


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    Structured Review

    Sino Biological sars-cov-2 (2019-ncov) nucleocapsid-his recombinant protein
    Sars Cov 2 (2019 Ncov) Nucleocapsid His Recombinant Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v08b/SARS-CoV-2+(2019-nCoV)+Nucleocapsid-His+recombinant+Protein/custom%4040588-v08b%4042521110
    Average 96 stars, based on 247 article reviews
    sars-cov-2 (2019-ncov) nucleocapsid-his recombinant protein - by Bioz Stars, 2026-09
    96/100 stars

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    Recombinant:

    Article Title: SARS-CoV-2 virus infection of Peromyscus leucopus demonstrates that infection tolerance is not limited to agents for which deermice are reservoirs
    Article Snippet: Purity of the recombinant protein was assessed as 95% by SDS-PAGE followed by staining with Simple Blue (Thermo Fisher Scientific). .. Recombinant nucleocapsid N protein was obtained from SinoBiological (USA) catalog 40588-v08b. .. Flat bottoms of 96-well ELISA plates (Corning; 3690) were coated overnight at 4°C with either recombinant S protein (100 ng per well) or nucleocapsid (N) protein (50 ng per well) in DPBS.

    Article Title: Peptide with neutralizing activity against severe acute respiratory syndrome coronavirus 2
    Article Snippet: A549 cells cultured for one day were washed with DMEM medium, and then 900 μL of recombinant SARS-CoV-2 spike RBD/peptide mixture was added to the cells. .. 500 ng of recombinant SARS-CoV-2 spike RBD His (Sino Biological, Cat. No.: 40592-V08B, China) was mixed with a peptide solution at each concentration in 1 mL of DMEM medium and reacted at room temperature for 1 hour. .. A549 cells cultured for one day were washed with DMEM medium, and then 900 μL of recombinant SARS-CoV-2 spike RBD/peptide mixture was added to the cells.

    Article Title: Peptide with neutralizing activity against severe acute respiratory syndrome coronavirus 2
    Article Snippet: 100 ng of recombinant SARS-CoV-2 spike RBD His (Sino Biological, Cat. No.: 40592-V08B, China) was mixed with a peptide solution at each concentration in PBS and reacted at room temperature for 1 hour. .. 100 ng of recombinant SARS-CoV-2 spike RBD His (Sino Biological, Cat. No.: 40592-V08B, China) was mixed with a peptide solution at each concentration in PBS and reacted at room temperature for 1 hour. ..

    Concentration Assay:

    Article Title: Peptide with neutralizing activity against severe acute respiratory syndrome coronavirus 2
    Article Snippet: A549 cells cultured for one day were washed with DMEM medium, and then 900 μL of recombinant SARS-CoV-2 spike RBD/peptide mixture was added to the cells. .. 500 ng of recombinant SARS-CoV-2 spike RBD His (Sino Biological, Cat. No.: 40592-V08B, China) was mixed with a peptide solution at each concentration in 1 mL of DMEM medium and reacted at room temperature for 1 hour. .. A549 cells cultured for one day were washed with DMEM medium, and then 900 μL of recombinant SARS-CoV-2 spike RBD/peptide mixture was added to the cells.

    Article Title: Peptide with neutralizing activity against severe acute respiratory syndrome coronavirus 2
    Article Snippet: 100 ng of recombinant SARS-CoV-2 spike RBD His (Sino Biological, Cat. No.: 40592-V08B, China) was mixed with a peptide solution at each concentration in PBS and reacted at room temperature for 1 hour. .. 100 ng of recombinant SARS-CoV-2 spike RBD His (Sino Biological, Cat. No.: 40592-V08B, China) was mixed with a peptide solution at each concentration in PBS and reacted at room temperature for 1 hour. ..

    Incubation:

    Article Title: An HLAII-targeted DNA vaccine against influenza H7N9 protected mice and ferrets from a virus challenge
    Article Snippet: .. HA [A/Shanghai/1/2013 (H7N9): 40104-V08B; A/Shanghai/2/2013 (H7N9): 40239-V08B, both from Sino Biological Inc.], blocked with 1% BSA, and incubated with serially diluted serum samples. .. HA-specific antibodies in mice were detected with alkaline phosphatase conjugated goat anti-mouse IgG (A1418, Sigma-Aldrich), and with alkaline phosphatase conjugated anti-ferret IgG (LS-C61240, LSBio, WA, US) in ferrets.

    Article Title: An HLAII-targeted DNA vaccine against influenza H7N9 protected mice and ferrets from a virus challenge.
    Article Snippet: .. HA [A/Shanghai/1/2013 (H7N9): 40104-V08B; A/Shanghai/2/2013 (H7N9): 40239-V08B, both from Sino Biological Inc.], blocked with 1% BSA, and incubated with serially diluted serum samples. .. HA-specific antibodies in mice were detected with alkaline phosphatase conjugated goat anti-mouse IgG (A1418, Sigma-Aldrich), and with alkaline phosphatase conjugated antiferret IgG (LS-C61240, LSBio, WA, US) in ferrets.

    other:

    Article Title: Affinity maturation and light-chain-mediated paratope diversification anticipates viral evolution
    Article Snippet: SARS-CoV-2 S2 , Sino Biological , Cat# 40590-V08B.



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